Journal: The Journal of Physiology

Article Title: Remuscularization of the failing heart

doi: 10.1113/JP273098

Figure Lengend Snippet: Schematic overview of strategies aiming at the remuscularization of the failing heart either by activation of endogenous mechanisms or by cardiomyocyte supplementation.

Article Snippet: Future strategies for enhanced therapeutic efficacy of cardiomyocyte grafts Despite some evidence for efficacy of tissue‐engineered and directly injected cardiomyocyte grafts in rat (Zimmermann et al . 2006 , Riegler et al . 2015 ; Qin et al . 2016 ), mouse (Didié et al . 2013 ), guinea pig (Weinberger et al . 2016 ) and macaque (Shiba et al . 2016 ) models, there is a clear need for an improvement of graft size.

Techniques: Activation Assay

Journal: Experimental and Therapeutic Medicine

Article Title: Plumbagin protects H9c2 cardiomyocytes against TBHP-induced cytotoxicity by alleviating ROS-induced apoptosis and modulating autophagy

doi: 10.3892/etm.2022.11428

Figure Lengend Snippet: PLB protects H9c2 cells from cell death. (A) Cell viability. (B) LDH activity. (C) CK activity. (D) Chemical structure of PLB. Data are expressed as the mean ± SD; n=6. **P<0.01 vs. control; ##P<0.01 vs. TBHP; determined using one-way ANOVA with Tukey's post hoc analysis. PLB, plumbagin; LDH, lactate dehydrogenase; CK, creatine kinase; TBHP, tertiary butyl hydrogen peroxide; OD, optical density.

Article Snippet: Cell culture and treatments H9c2 cardiomyocytes were obtained from the American Type Culture Collection (ATCC: CRL-1446).

Techniques: Activity Assay, Control

Journal: Experimental and Therapeutic Medicine

Article Title: Plumbagin protects H9c2 cardiomyocytes against TBHP-induced cytotoxicity by alleviating ROS-induced apoptosis and modulating autophagy

doi: 10.3892/etm.2022.11428

Figure Lengend Snippet: PLB promotes the autophagy of H9c2 cells. (A) Representative western blots for LC3-II/LC3-I. (B) Densitometric analysis for the ratio of LC3-II/LC3-I. Data are expressed as the mean ± SD; n=3. *P<0.05 vs. control; #P<0.05 vs. TBHP; determined using one-way ANOVA with Tukey's post hoc analysis. PLB, plumbagin; LC3, microtubule-associated protein 1 light chain 3; TBHP, tertiary butyl hydrogen peroxide.

Article Snippet: Cell culture and treatments H9c2 cardiomyocytes were obtained from the American Type Culture Collection (ATCC: CRL-1446).

Techniques: Western Blot, Control

Journal: International Journal of Bioprinting

Article Title: Blood-derived biomaterials for tissue graft biofabrication by solvent-based extrusion bioprinting

doi:

Figure Lengend Snippet: 3D bioprinting of specific tissue constructs using bioinks functionalized with blood-derived biomaterials

Article Snippet: Kumar et al. (2019); ACS Biomater Sci Eng [ ] , 1 mL of the bioink: 990 μL of fibrinogen solution (60 mg/mL), 155 mg of gelatin- furfuryl at 25°C Heat it at 37 °C for 1 h with stirring, add Rose Bengal (10 μL) Polymerization: 50 units/mL thrombin, and 80 mM CaCl 2 , Human-induced pluripotent stem cell (iPSC)-derived cardiomyocytes (CM) Human CM AC 16 cell lines (ATCC) , Fibrinogen Thrombin (#) , SEM, swelling assay, rheology, Fourier transform infrared spectroscopy (FTIR), biocompatibility Cardiac cell-laden constructs To validate the applicability of this scaffold toward mimicking cardiac tissue.

Techniques: Construct, Formulation, Migration, In Vivo, Clinical Proteomics, In Vitro, Micro-CT, Concentration Assay, Gene Expression, Transplantation Assay, Activation Assay, Immunohistochemistry, Immunohistochemical staining, Fourier Transform Infrared Spectroscopy, Spectroscopy, Functional Assay, In Situ, Modification, Activity Assay, Expressing, Marker, Comparison

Journal: Nature Communications

Article Title: Non-invasive detection of human cardiomyocyte death using methylation patterns of circulating DNA

doi: 10.1038/s41467-018-03961-y

Figure Lengend Snippet: Identification of cardiomyocyte-specific DNA methylation markers. a Unmethylation levels of FAM101A locus in 27 human tissues, including left ventricle, right ventricle and right atrium (red). Data representing methylation status of all CpG sites in the locus was extracted from the Roadmap Epigenomics Consortium browser. b Structure of the FAM101A locus, used as two independent markers: FAM101A and FAM101A AS. Lollipops represent CpG sites; arrows mark positions of PCR primers; S sense marker, AS antisense marker. c Demethylated FAM101A and FAM101A AS in DNA from multiple tissues and from isolated cardiomyocytes (purchased from ScienCell Research Laboratories, San Diego, CA). See online methods for an explanation why targeted PCR yields a lower background in non-cardiac tissues compared with the Roadmap browser in panel a . d Spike in experiments for FAM101A (left) and FAM101A AS (right). Human cardiomyocyte DNA was mixed with human leukocyte DNA in the indicated proportions (0–100%), and the percentage of fully unmethylated FAM101A molecules (in which all CpG sites were converted by bisulfite) was determined

Article Snippet: Lollipops represent CpG sites; arrows mark positions of PCR primers; S sense marker, AS antisense marker. c Demethylated FAM101A and FAM101A AS in DNA from multiple tissues and from isolated cardiomyocytes (purchased from ScienCell Research Laboratories, San Diego, CA).

Techniques: DNA Methylation Assay, Methylation, Marker, Isolation

Journal: Nature Communications

Article Title: Non-invasive detection of human cardiomyocyte death using methylation patterns of circulating DNA

doi: 10.1038/s41467-018-03961-y

Figure Lengend Snippet: Detection of cardiac cfDNA using digital droplet PCR. a Schematic of approach for ddPCR-based detection of methylation status of multiple adjacent cytosines. A signal from two probes in the same droplet reflects lack of methylation in five adjacent cytosines in the same original DNA strand. b Signal from cardiomyocyte and leukocyte DNA based on individual or dual probes. Scoring only dual probe signals drastically reduces noise from leukocyte DNA. c Spike-in experiment assessing sensitivity and linearity of signal from cardiomyocyte DNA diluted in leukocyte DNA. The use of dual probe enhances linearity and reduces baseline signal. x axis shows both the % of cardiac DNA diluted into blood DNA, and the absolute number of cardiomyocte genomes present in each sample. d Measurement of cardiac cfDNA in plasma of healthy adult and patients with myocardial infarction. The use of dual probes reduces the baseline signal in healthy plasma. Horizontal lines represent average and standard deviation of cfDNA values among the samples in each group

Article Snippet: Lollipops represent CpG sites; arrows mark positions of PCR primers; S sense marker, AS antisense marker. c Demethylated FAM101A and FAM101A AS in DNA from multiple tissues and from isolated cardiomyocytes (purchased from ScienCell Research Laboratories, San Diego, CA).

Techniques: Methylation, Clinical Proteomics, Standard Deviation